Personal Project + Intro to CRISPR

Monday - Personal Project Brainstorming

Outline:

1. Showing R+D of personal project
   • Samples and comparisons and determining what sample type to move forward with
2. Brainstorming
   • Jotting down ideas for our own ideas or directions, listening to others’ ideas and seeing what comes up from that
3. Prolonging acid exposure
   • Started an experiment where I prolonged how long the oyster shells are soaked in an acid that would strip calcium. However, because the shells are pH

Tuesday - Personal Project Cont.

Outline:

1. Shell crushing
   • Using a mortar and pestle to extract different sizes of oyster shell for different uses

Wednesday - CRISPR Protocol

Outline:

1. Introduction to CRISPR method
   • Getting familiar with the protocol and preparing the necessary material

Thursday - Personal Project Cont.

Outline:

1. Prolonging processing method
   • Soaking oystershells in a new bottle of white vinegar everyday, alkalinity v acidity
2. Continuation of crushing oyster shells
   • More powder!
3. Anne’s last day as instuctor :(
   • Sad

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